Equipment

FACS Aria:

facs_aria_resize_1.jpgFACS Aria
-3 laser, 14 color sorter running BDFACS Diva software on XP
-can sort into 1.5 mL, 15 mL, or 96 well plates
-can sort up to 4 populations simultaneously

The system uses fiber optic guided solid state 405, 488nm and 635nm diode lasers along with fiber guided emission collections (five colors plus scatter off the 488nm, three colors off the 635nm line, and 6 off the 405nm line).

 

facs aria laser configuration

FACS Calibur

facs_aria_resize_1.jpgFACS Calibur:
-2 laser (blue and red), 4 color analyzer, running CellQuest Pro on MacOSX

FACS calibur parameters

 

 

 

 

Beckman-Coulter ViCell

beckman coulter vicell analyzerBeckman-Coulter ViCell:

-Is an automated cell viability analyzer using trypan blue exclusion staining combined with imaged-based size analysis.

A cell sample is loaded into the system (minimum 1.0 mL, but can be diluted and dilution factored into final result), and the instrument aspirates the sample, mixes with the trypan blue, and loads the sample into the chamber and records: cell count/mL (equivalnet of 100), percent viability, and size distribution. It then performs and clean cycle. Sample processing time is approximately 3.5 minutes per sample

CTL-ImmunoSpot S5 Core Analyzer

ctl immunospot core analyzerCTL-ImmunoSpot S5 Core Analyzer:

-is CTLs dedicated model that has been fine tailored for ELISPOT work in the 96 well format

-features high end optics and cameras permitting to counting objects as small as >25  micrometer in size which can be critical for certain ELISPOT assays, including TNF and perforin measurements

The S5 Core ideally lends itself for GLP and high-throughput upgrades. The S5 Core instrument is ideally suited for users who do one particular type of assay while having the highest expectations for image and analysis quality.

Luminex 200

Luminex 200Luminex 200:

-an analyzer based on the principles of flow cytometry

-the system enables simultaneous measurement of up to 100 analytes in a few microliters of a single sample

Guava EasyCyte Plus:

Guava EasyCyte PlusGuava EasyCyte Plus:

-1 laser (blue) 3 color automated analyzer with built in 96 well and 10 tube format

 

Faxitron Compact 43855D Model

Faxitron compact 43855D modelFaxitron Compact 43855D Model:

-x-ray irradiation system has shielded cabinets and utilizes redundant safety interlocks

it is certified to comply with standards set by the U.S. Food and Drug Administration Center for Devices and Radiological Health, (21 CFR-1020.40)

-the system can be utilized in wide-ranging applications including feeder cell irradiation

Faxitron x-ray irradiation system provides a smaller, safer, simpler, and less costly alternative to radioisotrope irradiators for radiation exposure

PerkinElmer Victor X3

perkin elemer victor x3PerkinElmer Victor X3

VICTOR X3 is a multilabel plate reader, which includes Luminescence, Fluorescence Intensity (both top and bottom reading), and UV-absorbance (UV-VIS) technologies.

JABSOM ThermoFisher Attune Nxt

JABSOM Thermofisher Attune NXTJABSOM ThermoFisher Attune NXT

Acoustic focusing cytometer with 4 lasers (405, 488, 561 and 638nm) and 14 fluorescent parameters, running Attune software version 4.2 on Windows 10.

 


Can analyze samples from multiple tube formats as well as 96 (traditional and deep well) and 384 well plates. Capable of volumetric cell count, rare event detection, high volume samples acquisition, up to 10x faster (no wash, no lyse blood samples), and handles larger cells and particles (up to 300um) as well as clumpy cell samples. Equipped with external fluidics supply system to allow running large number of samples without needing to refill and/or empty fluids. We also have the ability to detect exosomes/small vesicles with the special small particle detector filters.

ThermoFisher Countess II FL

ThermoFisher Countess II FL

Countess II FL automated cell counter is the only counter with the option of reusable or disposable slides. It has fluorescence and brightfield capabilities that can count cells, monitor fluorescent protein expression and measure cell viability. EVOS light cubes include interchangable GFP, RFP, and DAPI.

NanoDrop 2000c

NanoDrop 2000c

The Thermo Scientific NanoDrop 2000c is a full-spectrum spectrophotometer that measures up to 2 ul samples with high accuracy and reproducibility. This system offers the convenience of both the NanoDrop patented sample retention technology and a traditional cuvette for sample measurements. The 2000c can measure high-concentration samples without dilution that many standard cuvette spectrophotometers cannot, measuring up to 200 times more concentrated solutions. Applications include measuring: Nucleic Acid Quantification, DNA Quantification, RNA Quantification, and Protein Quantification.