Home UH Manoa

News & Research Highlights
17 October 2014: Dengue Virus Assembly, prM Protein Cleavage and Entry

The precursor membrane (prM) protein of dengue virus (DENV) is present on the surface of immature virions. The role of nine highly conserved residues in the alpha-helical domain (MH) of the prM protein in DENV replication was investigated by alanine-substitution mutagenesis. Five mutants affected th…

02 October 2014: Dengue Virus Serotype Cross-Reactive Antibodies

The increased risk of severe dengue during repeat DENV infections appear to be due, at least in part, to the ability of pre-existing cross-reactive antibodies to form virus-antibody complexes and infect Fc-gamma receptor-bearing cells, known as antibody-dependent enhancement (ADE). The antigens and …

29 July 2014: West Nile Virus Neuroinvasive Disease

To identify the mechanisms by which West Nile virus (WNV) causes disruption of the blood-brain barrier (BBB) and leukocyte migration into the brain, the role of endothelial cell adhesion molecules (CAMs) was studied in an in vitro BBB model. Permeability of the BBB model increased following leukocyt…

View more ...

Recent Publications
PLoS Pathogens, 2014, Dengue viruses are enhanced by distinct populations of serotype cross-reactive antibodies in human immune sera.
de Alwis R, Williams KL, Schmid MA, Lai CY, Patel B, Smith SA, Crowe JE, Wang WK, Harris E, de Silva AM.

Dengue viruses (DENV) are mosquito-borne flaviviruses of global importance. DENV exist as four serotypes, DENV1-DENV4. Following a primary infection, individuals produce DENV-specific antibodies that bind only to the serotype of infection and other antibodies that cross-react with two or more seroty…

Journal of Biological Chemistry, 2014, Highly conserved residues in the helical domain of dengue virus type 1 precursor membrane protein are involved in assembly, prM protein cleavage and entry.
Hsieh SC, Wu YC, Zou G, Nerurkar VR, Shi PY, Wang WK.

The envelope and precursor membrane (prM) proteins of dengue virus (DENV) are present on the surface of immature virions. During maturation, prM protein is cleaved by furin protease into pr peptide and membrane (M) protein. While previous studies mainly focusing on the pr region have identified seve…

PLoS One, 2014, Blocking phosphatidylcholine utilization in Pseudomonas aeruginosa, via mutagenesis of fatty acid, glycerol and choline degradation pathways, confirms the importance of this nutrient source in vivo.
Sun Z, Kang Y, Norris MH, Troyer RM, Son MS, Schweizer HP, Dow SW, Hoang TT.

Pseudomonas aeruginosa can grow to very high-cell-density (HCD) during infection of the cystic fibrosis (CF) lung. Phosphatidylcholine (PC), the major component of lung surfactant, has been hypothesized to support HCD growth of P. aeruginosa in vivo. The phosphorylcholine headgroup, a glycerol molec…

View more ...
Seminars & Events
19 November 2014, 12:00 PM - 1:00 PM
Rosanna 'Anolani Alegado, Ph.D.

Bacteria as Drivers of Animal Evolution

17 September 2014, 12:00 PM - 1:30 PM
(1) Axel T. Lehrer, PhD, Asst Professor, Dept of Tropical Medicine, JABSOM; (2) John M. Berestecky, PhD, Professor of Microbiology, Kapiolani Community College; (3) Erlaine F. Bello, MD, Associate Professor, Dept of Medicine, JABSOM

RESEARCH COLLOQUIUM: “Ebola - What Do We Know?”

08 August 2014, 3:45 PM - 4:45 PM
Jonathan Mermin, MD, MPH

CANCELLED DUE TO HURRICANE ISELLE HIV, Viral Hepatitis, STD, andTB Prevention Today: What are the Big Unanswered Questions?

View more ...

15 March 2013, 1:00 PM - 3:30 PM
Robert Bowers, Ph. D.

Using QIIME for Microbial Community Analysis

13 November 2012, 9:00 AM - 5:00 PM
Pawel Michalak, Ph.D.


08 November 2012, 1:00 PM
Mirja Wine, Ph.D.

Considerations for Monitoring Immune Cells by Flow Cytometry, Signal Transduction Pathways, Intracellular Proteins, and Tetramers

View more ...

Recent Grants
NIH/NIAID, R01AI110769 Mature Virus-like Particles as a New Strategy for Dengue Virus Vaccines
04/01/2014 - 03/31/2019
NIH/NIAID, R21AI105286 Defining Immunity to Placental Malaria using a Multi-assay Predictive Model
03/01/2014 - 02/28/2016
NIH/NIMHD, T37MD008636 International Biomedical Research Training for Hawaiian & Pacific Island Students
12/01/2013 - 11/30/2018
View more ...