Implications Of Relative Fluorochrome Brightness In Resolving Your Target Populations & The Significance Of Antigen Density In Choosing The Appropriate Antibody/Color Combination & The Power of INDEX Sorting In Single Cell Research

Date/Time: 15 September 2016, 10:00 AM
Speaker: Lisa Bellemare, Ph.D.
Speaker Affiliation: Technical Applications Specialist, BD Biosciences
Venue: John A. Burns School of Medicine, Cancer Center Building, Room 401

For more info: Alex Gurary, Ph.D. (692-1794 or gurary@hawaii.edu)
Description: The Past 40 Years Has Seen Amazing Advancements In Flow Cytometry Technology, As We Are Seeing Instruments Capable Of 18-Color Analysis Become Standard. However, Multicolor Panel Design Until Now Has Been Optimized Largely By Trial And Error. Based On Our Current Findings With The Newest Fluorochrome Technology, We Will Share An Approach To Panel Design That Enables Scientists To Unlock The Full Potential Of Their Research Offered By Innovations In Flow Cytometry Systems.

The MCI Core’s BD FACSAria Sorter Just Underwent An Upgrade: We Are Now Able To Execute INDEX Sorting. INDEX Sorting Is The Ability To Sort Single Cells Into 96 well/PCR Plates And Allowing One To Record The Fluorescence Levels Of The One Sorted Cell, And Tie That Data With Any Downstream Application. We Will Explain The Capabilities And Possibilities Of Such A Powerful Tool In Single Cell Research.

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