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Stable, site-specific fluorescent tagging constructs optimized for Burkholderia species.

Norris MH, Kang Y, Wilcox B, Hoang TT.

Citation

Norris MH, Kang Y, Wilcox B, Hoang TT. (2010) Stable, site-specific fluorescent tagging constructs optimized for Burkholderia species. Applied and Environmental Microbiology 76(22):7635-7640.


Abstract

Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.


Link: http://www.ncbi.nlm.nih.gov/pubmed/20851961
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