Richard J. Kuhn, Ph.D.

Selected Publications

• Grabowski JM, Perera R, Roumani AM, Hedrick VE, Inerowicz HD, Hill CA, Kuhn RJ.

  Changes in the proteome of Langat-infected Ixodes scapularis ISE6 cells: Metabolic pathways associated with flavivirus infection.

  PLoS Neglected Tropical Diseases 2016 more details...
  
• Gulia-Nuss M, et al.

  Genomic insights into the Ixodes scapularis tick vector of Lyme disease.

  Nature Communications 2016 more details...
  
• Akey DL, Brown WC, Jose J, Kuhn RJ, Smith JL.

  Structure-guided insights on the role of NS1 in flavivirus infection.

  Bioessays 2015 more details...
  
• D'Aquila T, Sirohi D, Grabowski JM, Hedrick VE, Paul LN, Greenberg AS, Kuhn RJ, Buhman KK.

  Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge.

  PLoS One 2015 more details...
  
• Jose J, Tang J, Taylor AB, Baker TS, Kuhn RJ.

  Fluorescent protein-tagged Sindbis virus E2 glycoprotein allows single particle analysis of virus budding from live cells.

  Viruses 2015 more details...
  
• Kuhn RJ, Dowd KA, Beth Post C, Pierson TC.

  Shake, rattle, and roll: Impact of the dynamics of flavivirus particles on their interactions with the host.

  Virology 2015 more details...
  
• Liu Y, Sheng J, Fokine A, Meng G, Shin WH, Long F, Kuhn RJ, Kihara D, Rossmann MG.

  Structure and inhibition of EV-D68, a virus that causes respiratory illness in children.

  Science 2015 more details...
  
• Yan R, Edwards TJ, Pankratz LM, Kuhn RJ, Lanman JK, Liu J, Jiang W.

  A fast cross-validation method for alignment of electron tomography images based on Beer-Lambert law.

  Journal of Structural Biology 2015 more details...
  
• Yan R, Edwards TJ, Pankratz LM, Kuhn RJ, Lanman JK, Liu J, Jiang W.

  Simultaneous determination of sample thickness, tilt, and electron mean free path using tomographic tilt images based on Beer-Lambert law.

  Journal of Structural Biology 2015 more details...
  
• Zhang X, Sheng J, Austin SK, Hoornweg TE, Smit JM, Kuhn RJ, Diamond MS, Rossmann MG.

  Structure of acidic pH dengue virus showing the fusogenic glycoprotein trimers.

  Journal of Virology 2015 more details...
  
• Aggarwal M, Dhindwal S, Kumar P, Kuhn RJ, Tomar S.

  trans-Protease activity and structural insights into the active form of the alphavirus capsid protease.

  Journal of Virology 2014 more details...
  
• Akey DL, Brown WC, Dutta S, Konwerski J, Jose J, Jurkiw TJ, DelProposto J, Ogata CM, Skiniotis G, Kuhn RJ, Smith JL.

  Flavivirus NS1 structures reveal surfaces for associations with membranes and the immune system.

  Science 2014 more details...
  
• Apte-Sengupta S, Sirohi D, Kuhn RJ.

  Coupling of replication and assembly in flaviviruses.

  Current Opinion in Virology 2014 more details...
  
• Dowd KA, Mukherjee S, Kuhn RJ, Pierson TC.

  Combined effects of the structural heterogeneity and dynamics of flaviviruses on antibody recognition.

  Journal of Virology 2014 more details...
  
• Junjhon J, Pennington JG, Edwards TJ, Perera R, Lanman J, Kuhn RJ.

  Ultrastructural characterization and three-dimensional architecture of replication sites in dengue virus-infected mosquito cells.

  Journal of Virology 2014 more details...
  
• Plevka P, Lim PY, Perera R, Cardosa J, Suksatu A, Kuhn RJ, Rossmann MG.

  Neutralizing antibodies can initiate genome release from human enterovirus 71.

  Proceedings of the National Academy of Sciences USA 2014 more details...
  
• Catanese MT, Uryu K, Kopp M, Edwards TJ, Andrus L, Rice WJ, Silvestry M, Kuhn RJ, Rice CM.

  Ultrastructural analysis of hepatitis C virus particles.

  Proceedings of the National Academy of Sciences U S A 2013 more details...
  
• Dai HS, Liu Z, Jiang W, Kuhn RJ.

  Directed evolution of a virus exclusively utilizing human epidermal growth factor receptor as the entry receptor.

  Journal of Virology 2013 more details...
  
• Keating JA, Bhattacharya D, Lim PY, Falk S, Weisblum B, Bernard KA, Sharma M, Kuhn RJ, Striker R.

  West Nile virus methyltransferase domain interacts with protein kinase G.

  Virology Journal 2013 more details...
  
• Plevka P, Perera R, Yap ML, Cardosa J, Kuhn RJ, Rossmann MG.

  Structure of human enterovirus 71 in complex with a capsid-binding inhibitor.

  Proceedings of the National Academy of Sciences U S A 2013 more details...
  
• Zhang W, Kaufmann B, Chipman PR, Kuhn RJ, Rossmann MG.

  Membrane curvature in flaviviruses.

  Journal of Structural Biology 2013 more details...
  
• Zhang X, Sheng J, Plevka P, Kuhn RJ, Diamond MS, Rossmann MG.

  Dengue structure differs at the temperatures of its human and mosquito hosts.

  Proceedings of the National Academy of Sciences USA 2013 more details...
  
• Battisti AJ, Yoder JD, Plevka P, Winkler DC, Prasad VM, Kuhn RJ, Frey TK, Steven AC, Rossmann MG.

  Cryo-electron tomography of rubella virus.

  Journal of Virology 2012 more details...
  
• Jose J, Przybyla L, Edwards TJ, Perera R, Burgner JW, Kuhn RJ.

  Interactions of the cytoplasmic domain of Sindbis virus E2 with nucleocapsid cores promote alphavirus budding.

  Journal of Virology 2012 more details...
  
• Perera R, Riley C, Isaac G, Hopf-Jannasch AS, Moore RJ, Weitz KW, Pasa-Tolic L, Metz TO, Adamec J, Kuhn RJ.

  Dengue virus infection perturbs lipid homeostasis in infected mosquito cells.

  PLoS Pathogens 2012 more details...
  
• Pierson TC, Kuhn RJ.

  Capturing a virus while it catches its breath.

  Structure 2012 more details...
  
• Plevka P, Perera R, Cardosa J, Kuhn RJ, Rossmann MG.

  Structure determination of enterovirus 71.

  Acta Crystallogr D Biol Crystallogr 2012 more details...
  
• Plevka P, Perera R, Cardosa J, Kuhn RJ, Rossmann MG.

  Crystal structure of human enterovirus 71.

  Science 2012 more details...
  
• Snyder JE, Berrios CJ, Edwards TJ, Jose J, Perera R, Kuhn RJ.

  Probing the early temporal and spatial interaction of the Sindbis virus capsid and E2 proteins with reverse genetics.

  Journal of Virology 2012 more details...
  
• Aggarwal M, Dhindwal S, Pratap S, Kuhn RJ, Kumar P, Tomar S.

  Crystallization, high-resolution data collection and preliminary crystallographic analysis of Aura virus capsid protease and its complex with dioxane.

  Acta Crystallographica. Sect F, Structural Biology and Crystallization Communications 2011 more details...
  
• Gualtieri EJ, Guo F, Kissick DJ, Jose J, Kuhn RJ, Jiang W, Simpson GJ.

  Detection of membrane protein two-dimensional crystals in living cells.

  Biophysics Journal 2011 more details...
  
• Khadka S, Vangeloff AD, Zhang C, Siddavatam P, Heaton NS, Wang L, Sengupta R, Sahasrabudhe S, Randall G, Gribskov M, Kuhn RJ, Perera R, LaCount DJ.

  A physical interaction network of dengue virus and human proteins.

  Molecular and Cellular Proteomics 2011 more details...
  
• Mayhoub AS, Khaliq M, Botting C, Li Z, Kuhn RJ, Cushman M.

  An investigation of phenylthiazole antiflaviviral agents.

  Bioorganic and Medicinal Chemistry 2011 more details...
  
• Mayhoub AS, Khaliq M, Kuhn RJ, Cushman M.

  Design, synthesis, and biological evaluation of thiazoles targeting flavivirus envelope proteins.

  Journal of Medicinal Chemistry 2011 more details...
  
• Plevka P, Battisti AJ, Junjhon J, Winkler DC, Holdaway HA, Keelapang P, Sittisombut N, Kuhn RJ, Steven AC, Rossmann MG.

  Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres.

  EMBO Reports 2011 more details...
  
• Snyder JE, Azizgolshani O, Wu B, He Y, Lee AC, Jose J, Suter DM, Knobler CM, Gelbart WM, Kuhn RJ.

  Rescue of infectious particles from preassembled alphavirus nucleocapsid cores.

  Journal of Virology 2011 more details...
  
• Tang J, Jose J, Chipman P, Zhang W, Kuhn RJ, Baker TS.

  Molecular links between the E2 envelope glycoprotein and nucleocapsid core in Sindbis virus.

  Journal of Molecular Biology 2011 more details...
  
• Tomar S, Narwal M, Harms E, Smith JL, Kuhn RJ.

  Heterologous production, purification and characterization of enzymatically active Sindbis virus nonstructural protein nsP1.

  Protein Expression and Purification 2011 more details...
  
• Heaton NS, Perera R, Berger KL, Khadka S, Lacount DJ, Kuhn RJ, Randall G.

  Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis.

  Proceedings of the National Academy of Sciences USA 2010 more details...
  
• Junjhon J, Edwards TJ, Utaipat U, Bowman VD, Holdaway HA, Zhang W, Keelapang P, Puttikhunt C, Perera R, Chipman PR, Kasinrerk W, Malasit P, Kuhn RJ, Sittisombut N.

  Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles.

  Journal of Virology 2010 more details...
  
• Kaufmann B, Plevka P, Kuhn RJ, Rossmann MG.

  Crystallization and preliminary X-ray diffraction analysis of West Nile virus.

  Acta Crystallographica Section F: Structural Biology and Crystallization Communications 2010 more details...
  
• Kaufmann B, Vogt MR, Goudsmit J, Holdaway HA, Aksyuk AA, Chipman PR, Kuhn RJ, Diamond MS, Rossmann MG.

  Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354.

  Proceedings of the National Academy of Sciences USA 2010 more details...
  
• Li L, Jose J, Xiang Y, Kuhn RJ, Rossmann MG.

  Structural changes of envelope proteins during alphavirus fusion.

  Nature 2010 more details...
  
• Yu IM, Holdaway HA, Chipman PR, Kuhn RJ, Rossmann MG, Chen J.

  Association of the pr peptides with dengue virus at acidic pH blocks membrane fusion.

  Journal of Virology 2009 more details...