FLOW CYTOMETRY AND CELL SORTING FOR EVERY TYPE OF BIOLOGICAL SAMPLES

Date/Time: 06 June 2019, 10:00 AM
Speaker: Lisa Bellemare
Speaker Affiliation: Research Applications Scientist, BD Biosciences
Venue: University of Hawaii at Manoa, SOEST Video Wall, Room 131, Hawaii Institute of Geophysics (HIG), 2525 Correa Rd, Honolulu, HI 96822

For more info: Alexandra Gurary, 808-692-1794, gurary@hawaii.edu
Description: DURING THIS SEMINAR, THE BASICS OF FLOW CYTOMETRY AND CELL SORTING WILL BE COVERED
INCLUDING SAMPLE PREPARATION FOR A VARIETY TYPES OF SAMPLES: PLANKTON, BACTERIA, YEASTS, EUKARYOTIC AND PROKARYOTIC CELLS, AS WELL AS INSTRUMENT SETTINGS AND THE NECESSARY CORRECT CONTROLS. WE WILL ALSO DISCUSS THE TYPE OF DOWNSTREAM ANALYSIS POSSIBLE AFTER CELL SORTING AND THE ADVANTAGES OF INDEX SORTING, WHICH IS THE ABILITY TO SORT SINGLE CELLS INTO 96 WELL/PCR PLATES AND ALLOWING ONE TO RECORD THE FLUORESCENCE LEVELS OF THE ONE SORTED CELL, AND TIE THAT DATA WITH ANY DOWNSTREAM APPLICATION. WE WILL EXPLAIN THE CAPABILITIES AND POSSIBILITIES OF SUCH A POWERFUL TOOL IN SINGLE CELL RESEARCH.
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